Table 1.
Primers used in this study
| Description | Primer name | Sequence (5′ to 3′)a | Position | Reference |
|---|---|---|---|---|
| aj1-LP-fusB detection | ri18 250-269F | GTTGCTAAATCTCCTCACGG | ri18 250-269 | This study |
| 7778 LA 410-390R | GGGTAAATCCAGAGTTAATCG | fusB −232 to −252 | ||
| aj1 606-577R | AGTAAAGAATAAGTTTTTAATCGTTAATGC | aj1 606-577 | ||
| fusB 389-361R | TTCCGATTTGATGCAAGTTCATTCCATCC | fusB 389-361 | ||
| LA-PCR for HindIII fragments | fusB 437-465F | GAGAAATTTCTAATCAGGTTGTAAAGGGG | fusB 437-465 | This study |
| fusB 530-558F | CGGATGGTCAATATGTAAAAAAAGGTGAC | fusB 530-558 | ||
| fusB 553-580F | GGTGACTATATATGTCGAGATAGCATTC | fusB 553-580 | ||
| fusB 282-253R | AAGTTTTTGCGGACTAGGTAGTTCAAAAGG | fusB 282-253 | ||
| LA-PCR for PstI fragments | 7778 LA 755-736R | CGATTGAATAACTTTGACGG | fusB 114-95 | This study |
| 7778 upstream detection | S. epi rpsR 6-24F | AGGTGGACCAAGAAGAGGC | rpsR 6-24 | This study |
| 2793 upstream detection | S. epi groEL 1213-1232F | GTKGAAGAAGGTATYGTTGC | groEL 1213-1232 | This study |
| Prediction of insertion sites | int(I) 109-128F | CGTAAATCAGACGCTAAACA | SeRIfusB-2793int 109-128 | This study |
| int(I) 1139-1122R | CTAAACTTGTGGGAAGCG | SeRIfusB-2793int 1139-1122 | ||
| int(II) 541-565F | GCTAAACGTAATAACTATTTAGAAG | SeRIfusB-7778int 541-565 | ||
| int 1061-1042R | GTGTGACGTAATGTGTGCGT | SeRIfusB-7778int 1061-1042 | ||
| S. epi groEL 1582-1599F | GAACAACCTGGAATGGGT | groEL 1582-1599 | ||
| 185 LA 3R | CTCACAGAGGTTCTATAATGTTGG | SeRIfusB-2793 ORF19 419-396 | ||
| fusB LA-2R | AATACTCCTGGATGGCGT | SeRIfusB-7778 ORF19 −216 to −233 |
a
K = G or T; Y = C or T.