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Quantitative RT-PCR of RNAIII transcription at 2 h (A) and 24 h (B) in vitro and in infected cardiac vegetations in the IE model 24 h postinfection (C). Relative transcript levels of RNAIII were determined by RT-PCR. Results are presented as the ΔCT (gyrBCT − RNAIII CT) and represent the mean (+SD) of two biological replicates in vitro and of at least two animals in vivo. Isolates with strong delta-hemolysin activities are underlined.
