Table 1.

Strains used in this study

Source and strain	Relevant characteristicsa	VAN MIC (μg/ml)b	Reference(s)
Laboratory strains
RN4220	NCTC8325-4, α-hemolysin negative, β-hemolysin positive		33
RN6911	NCTC8325-4 agr::tet(M), Tcr		33
SH1000	rsbU-positive derivative of NCTC8325-4, agr-I		17
SH1001	SH1000 agr::tet(M), Tcr		17
Clinical MRSA bacteremia isolates
300-087	agr-I, SCCmec IV, CC45c	0.5	7, 37
324-136	agr-I, SCCmec IV, CC45c	0.5	7, 37
300-169	agr-I, SCCmec IV, CC45c	0.5	7, 37
300-103	agr-I, SCCmec IV, CC45c	0.5	7, 37
301-188	agr-I, SCCmec IV, CC45c	0.5	7, 37
300-246	agr-II, SCCmec I, CC5c	0.5	7, 37
010-016	agr-II, SCCmec II, CC5c	1	7, 37
077-107	agr-II, SCCmec II, CC5c	1	7, 37
088-180	agr-II, SCCmec II, CC5c	0.5	7, 37
088-237	agr-II, SCCmec II, CC5c	0.5	7, 37
Mutants
300-169Δagr	300-169 agr::tet(M), Tcr	0.5	This study
324-136Δagr	324-136 agr::tet(M), Tcr	0.5	This study

^aTc^r, tetracycline resistant.

^bVancomycin MICs were determined according to CLSI guidelines.

^cspa types were used to predict clonal complexes, according to the methods of McCalla et al. (24).