Fig. 1.

ClustalW alignment of ROP18_II, ROP2_I, and ROP8_I, showing the sequence included in the homology model. For ROP18_II, residue numbers are based on the first methionine of ToxoDB gene model TGME49_005250. Key residues are indicated by squares: M356 is the putative “gatekeeper” residue in the ATP binding pocket, D362 is the predicted catalytic aspartic acid, D427 and N414 coordinate interactions with Mg²⁺, K281 is predicted to bind the alpha and beta phosphates of ATP, and E300 is predicted to coordinate ATP positioning.