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. 2011 Dec;55(12):5459–5468. doi: 10.1128/AAC.05178-11

Fig. 1.

Fig. 1.

Construction of the A. lentulus cyp51A-defective mutant strain. (A) Design of the fusion vector for A. lentulus cyp51A gene deletion. The location of restriction sites used for the Southern analysis are indicated before (upper section) and after (lower section) the cyp51A locus. The transformants were selected using the hyg gene (1.4 kb) flanked by SalI (S) restriction sites included in the deletion cassette (striped bars). The excised cyp51A coding fragment and the probe used for hybridization are indicated in gray and black bars, respectively. The location of the primers for fusion vector construction is indicated in the middle section of the diagram. (B) Southern hybridization of genomic DNA of A. lentulus digested with SalI and XhoI (X). Lane 1, wild-type strain; lane 2, A. lentulus cyp51A-deficient T38.18 strain. Lanes 3 and 4 show two ectopic transformants that maintained the wild-type cyp51A copy. Sizes of the expected fragment are indicated in bp on the right side. Lane M, 1-kb molecular size marker.