Table 1.

Oligonucleotides used throughout this work

Name and group	Orientation	Sequence (5′-3′)	Purpose
A. lentuluscyp51A deletion
L-1	Sense	CACTGTTTCTCATACAGGGAAACCTCATGG	1st PCR, 5′-end vector construction; 2nd PCR, fusion vector
LH1	Sense	GTTTTCGGATCTGACGTGGTGTATGATTGTTCGTCGACGTTAACTGATATTGAAGGAGCA	Hygromycin cassette amplification
LH2	Antisense	TGCTCCTTCAATATCAGTTAACGTCGACGAACAATCATACACCACGTCAGATCCGAAAAC	5′-End vector construction
HL3	Sense	CAGTTAACGTCGACGAATTCGATATCAAGCGACCTCTATCATGATCTGGACAAGGGCTTT	Hygromycin cassette amplification
HL2	Antisense	AAAGCCCTTGTCCAGATCATGATAGAGGTCGCTTGATATCGAATTCGTCGACGTTAACTG	Hygromycin cassette amplification
L-2	Antisense	CCCTGAGAAGAGCGATGAATAGTCGGTATC	1st PCR, 3′-end vector construction; 2nd PCR, fusion vector
L-3	Sense	TTGTGCCTAGCAAGGAAAAAGACAAGAAA	PCR gene deletion verification
A. lentuluscyp51A expression in A. fumigatus
A	Sense	TAGAATGAGTGAGCTGATTTGCCATGGATT	1st PCR, 5′-end vector construction; 2nd PCR, fusion vector
B2	Antisense	GTAGGCCGTGAGCAATAGCATCGACACCATTTCGAGGAGACACAGGGAGGGTGAGCCCGA	5′-End vector construction
C2	Sense	TCGGGCTCACCCTCCCTGTGTCTCCTCGAAATGGTGTCGATGCTATTGCTCACGGCCTAC	Coding Cyp51A A. lentulus insertion
D3	Antisense	CCTTTGAAGTCCTCGATGGTTACAACAGTCTCACTTGGATGTGTCTTTAGAACGCTTCTC	Coding Cyp51A A. lentulus insertion
E3	Sense	GAGAAGCGTTCTAAAGACACATCCAAGTGAGACTGTTGTAACCATCGAGGACTTCAAAGG	A. fumigatus terminator insertion
F3	Antisense	TGCTCCTTCAATATCAGTTAACGTCGACGAGCGTGCCAAGGCCAAGGCTTGATTAAGTAT	A. fumigatus terminator insertion
G3	Sense	ATACTTAATCAAGCCTTGGCCTTGGCACGCTCGTCGACGTTAACTGATATTGAAGGAGCA	Hygromycin resistance cassette
F4	Antisense	CTGTTCAGTCAGGGCATGTCCTTGGAGACTAAGCTTGATATCGAATTCGTCGACGTTAAC	Hygromycin resistance cassette
G4	Sense	GTTAACGTCGACGAATTCGATATCAAGCTTAGTCTCCAAGGACATGCCCTGACTGAACAG	3′-End vector construction
H2	Antisense	AGAGATAGAACCTTGGAGTCTGCTTGCCTC	1st PCR, 3′-end vector construction; 2nd PCR, fusion vector
A. lentuluscyp51A expression assessment
Len-A4	Sense	CAGACATGATTTGGAACC	cDNA amplification
Len-Cypa2	Antisense	TTAGAACGCTTCTCCCAG	cDNA amplification
A. fumigatuscyp51A expression analysis
A4	Sense	CAGACATGATATGGAACC	cDNA amplification
Cypa2	Antisense	TTCGACCGCTTCTCCCAG	cDNA amplification
Tub5	Sense	TGACCCAGCAGATGTT	House-keeping gene used as reference for normalization
Tub6	Antisense	GTTGTTGGGAATCCACTC	House-keeping gene used as reference for normalization