Table 2.

Oligonucleotide primers for quantitative RT-PCR^a

Primer	Sequence (5′ → 3′)	Tm (°C)	Target gene
Ldh for (HKG)*	GCC GCA GTA AAG AAC TTG ATG	57.9	OEOE_0413
Ldh rev*	TGC CGA CAA CAC CAA CTG TTT	57.9	OEOE_0413
gyrA for (HKG)*	CGC CCG ACA AAC CGC ATA AA	59.4	OEOE_0006
gyrA rev*	CAA GGA CTC ATA GAT TGC CGA A	58.4	OEOE_0006
829 Beacon for	CTC CCC GCT GGC TGA TTA TG	61.4	OEOE_0478
829 Beacon rev	TTG AGT GAC TTC TTG TTG GCT TTG	59.3	OEOE_0478
Ooe 839-5	CGC CAA TCA GCA GCT ATA TTA TG	58.9	OEOE_0819
Ooe 839-6	GAA TGA CTA GTG GTG CAA GAA C	58.4	OEOE_0819
Ooe 1047-1	GGA GCC TTC CTT GTA AAC TGG	59.8	OEOE_1574
Ooe 1047-2	CCT TCA TTT AAC AGC TGT CGT GG	60.6	OEOE_1574
1719 for	CCA TCA GGG AAT TGT CAG TCC	59.8	OEOE_1766
1719 rev	TAA GCA AAG GAT ATA TCA GGC TG	57.1	OEOE_1766
1349 for	ATT GTT ATC ACC TGT ACG ATG CTG	59.3	OEOE_1681
1349 rev	CAA CGC CGA AGT CCA TTA TCC	59.8	OEOE_1681
1629 for	TTT TAC TTT TGC CCG TCC AG	55.3	OEOE_1682
1629 rev	TGC CGA CTG AGA TCC AGA TA	57.3	OEOE_1682
E1 for	TTA TTG GAA ATT GCC GAA GG	53.2	OEOE_0647
E1 rev	TTT CTT CGC TTG ACG GAA CT	55.3	OEOE_0647
Hpr for	GCG ACG ATG CTT GTT CAA A	54.5	OEOE_0643
Hpr rev	GCG CCA AGA CTC ATT ACA C	56.7	OEOE_0643
414 for	CCA AGT GAG CTG CAA CAA AA	55.3	OEOE_0382
414 rev	GGC AGC CAA TAT CCT AGT CG	59.4	OEOE_0382
1138 for	GTG TTT CGA TGA TCG CTT CC	57.3	OEOE_0296
1138 rev	CGT AAA ACC TTC CCC ATG CAA A	58.4	OEOE_0296
808 for	GGA AAT TGC CAC TCA TAT AAT CG	57.1	OEOE_0464
808 rev	GAA TCC ACT CGA GCC AAA AC	57.3	OEOE_0464
136 Beacon for	ATC GTT CTT GTC ATT GGT GAG ATG	59.3	OEOE_0136
136 Beacon rev	CAT TCC CTG TCC TTG AAA CTG	60.3	OEOE_0136
1706 for	GCA ACA TCA AAA ACA GAA CCA A	54.7	OEOE_1706
1706 rev	TAA GCG CAT CTC CGA AGT TT	55.3	OEOE_1706
1714 for	GGA CAA CAA TTC AGA TGT GG	55.3	OEOE_1714
1714 rev	GCA TGT AGC CGA TTG TTG AA	55.3	OEOE_1714

^aLabeled primers (*) are the same as in reference 6. The target gene gives the gene number as in the O. oeni database (www.doe.jgi.gov).