Table 1.
Strains and plasmids used in this study
| Strain or plasmida | Genotype or phenotype | Reference |
|---|---|---|
| Strains | ||
| W3110 | F− IN(rrnD-rrnE)1 | 18 |
| RA1017 | W3110 ΔexbBD::kan ΔtolQRA | 29 |
| RA1045 | W3110 ΔexbD ΔtolQR | 5 |
| KP1344 | W3110 tonB P14::blaM | 33 |
| KP1509 | W3110 ΔexbD ΔtolQR ΔtonB::kan | 38 |
| Plasmids | ||
| pKP381 | TonB(H20A) | 29 |
| pKP945 | TonB(C18G, A150C) | 38 |
| pKP660 | exbB exbD in pBAD24 | 38 |
| pKP885 | ExbB ExbD(K97C) | Present study |
| pKP899 | ExbB ExbD(F103C) | Present study |
| pKP905 | ExbB ExbD(T109C) | Present study |
| pKP911 | ExbB ExbD(L115C) | Present study |
| pKP1000 | ExbD(A92C) | 38 |
| pKP1049 | ExbD(D25N, A92C) | 38 |
| pKP999 | exbD in pPro24 | 38 |
| pKP1001 (L93C) | ||
| pKP1002 (T94C) | ||
| pKP1003 (E95C) | ||
| pKP1004 (G96C) | ||
| pKP1005 (K97C) | ||
| pKP1006 (K98C) | ||
| pKP1007 (D99C) | ||
| pKP1008 (T100C) | ||
| pKP1009 (T101C) | ||
| pKP1010 (I102C) | ||
| pKP1011 (F103C) | ||
| pKP1012 (F104C) | ||
| pKP1013 (R105C) | ||
| pKP1014 (A106C) | ||
| pKP1015 (D107C) | ||
| pKP1016 (K108C) | ||
| pKP1017 (T109C) | ||
| pKP1018 (V110C) | ||
| pKP1019 (D111C) | ||
| pKP1020 (Y112C) | ||
| pKP1021 (E113C) | ||
| pKP1022 (T114C) | ||
| pKP1023 (L115C) | ||
| pKP1024 (M116C) | ||
| pKP1025 (K117C) | ||
| pKP1026 (V118C) | ||
| pKP1027 (M119C) | ||
| pKP1028 (D120C) | ||
| pKP1029 (T121C) | ||
| pKP1064 | ExbD(D25N) | 38 |
| pKP1217 (D25N, L93C) | ||
| pKP1216 (D25N, T94C) | ||
| pKP1218 (D25N, E95C) | ||
| pKP1219 (D25N, G96C) | ||
| pKP1050 (D25N, K97C) | ||
| pKP1233 (D25N, K98C) | ||
| pKP1203 (D25N, D99C) | ||
| pKP1204 (D25N, T100C) | ||
| pKP1260 (D25N, T101C) | ||
| pKP1189 (D25N, I102C) | ||
| pKP1051 (D25N, F103C) | ||
| pKP1226 (D25N, F104C) | ||
| pKP1227 (D25N, R105C) | ||
| pKP1228 (D25N, A106C) | ||
| pKP1261 (D25N, D107C) | ||
| pKP1270 (D25N, K108C) | ||
| pKP1052 (D25N, T109C) | ||
| pKP1237 (D25N, V110C) | ||
| pKP1262 (D25N, D111C) | ||
| pKP1269 (D25N, Y112C) | ||
| pKP1234 (D25N, E113C) | ||
| pkP1274 (D25N, T114C) | ||
| pKP1053 (D25N, L115C) | ||
| pKP1429 (D25N, M116C) | ||
| pKP1290 (D25N, K117C) | ||
| pKP1291 (D25N, V118C) | ||
| pKP1294 (D25N, M119C) | ||
| pKP1297 (D25N, D120C) | ||
| pKP1082 (D25N, T121C) |
a
The plasmids listed below pKP999 and pKP1064 are derivatives of pKP999 and pKP1064, respectively, unless otherwise noted in Materials and Methods. The ExbD substitutions expressed from each plasmid are listed in parentheses.