Fig. 3.
Membrane and surface localization of the A. actinomycetemcomitans epithelial cell adhesin Aae. (A) Immunoblot analysis of bacterial membranes probed with a polyclonal antiserum specific for Aae. Lane 1, aae mutant strain; lane 2, wild-type (WT) strain (VT1169); lane 3, aae mutant strain transformed with a replicating plasmid expressing the EmaA signal peptide-Aae chimera (EmaASP-Aae). (B) Detection of Aae on the bacterial surface. Surface-exposed Aae was detected using antibodies specific for Aae in an ELISA format with bacteria immobilized on the bottom of wells on 96-well microtiter plates.