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. 2011 Dec;193(24):6983–6994. doi: 10.1128/JB.05813-11

Fig. 5.

Fig. 5.

Whole-mount transmission electron micrographs of A. actinomycetemcomitans strains negatively stained with Nano-W. Positive control, emaA mutant strain transformed with a plasmid encoding the intact EmaA signal peptide; EmaAΔ2-23, emaA mutant strain transformed with a plasmid encoding the EmaA signal peptide with deletion of amino acids 2 to 23; EmaAΔ24-53, emaA mutant strain transformed with a plasmid encoding the EmaA signal peptide with deletion of amino acids 24 to 53; EmaAΔ2-35, emaA mutant strain transformed with a plasmid encoding the EmaA signal peptide with deletion of amino acids 2 to 35; EmaAΔ16-39, emaA mutant strain transformed with a plasmid encoding the EmaA signal peptide with deletion of amino acids 16 to 39; Omp34SP-EmaA, emaA mutant strain transformed with a plasmid encoding the Omp34 signal peptide instead of the EmaA signal peptide; WT, wild-type strain transformed with empty plasmid; KM333, chromosomal deletion strain of EmaA amino acids 16 to 39; negative control, emaA mutant strain transformed with the empty plasmid. The small black arrows indicate EmaA structures on the bacterial surface.