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. 2011 Dec;193(23):6567–6575. doi: 10.1128/JB.05803-11

Fig. 3.

Fig. 3.

The production of anthranilate and PQS is decreased in the kynR mutant. Ethyl acetate extracts from cultures grown for 6 (A) and 24 h (B) were analyzed by TLC. Equal volumes of extracts were resolved in each lane, and the addition of water (W), 1 mM l-tryptophan (T), 1 mM l-kynurenine (K), or 1 mM anthranilic acid (A) to the respective culture is indicated at the top of each lane. Lane 1 contains 75 ng of PQS, and lane 18 contains 1.25 ng of anthranilic acid (controls). Lanes 2 to 5 contain extracts from strain PAO1 grown in LB; lanes 6 to 9 contain extracts from strain PAΔKynR grown in LB; lanes 10 to 13 contain extracts from strain PAO1 grown in VBG; lanes 14 to 17 contain extracts from strain PAΔKynR grown in VBG.