Fig. 5.

KynR can induce expression of kynB′-lacZ in the presence of l-kynurenine in E. coli. E. coli strain DH5α(pJF01)(pKynRex) was grown in LB medium in the presence of water as a control water (white bar), 1 mM l-kynurenine (black bar), 1 mM l-tryptophan (striped bar), or 1 mM anthranilate (gray bar). After 3 h of growth the cultures were induced with 0.1% arabinose and grown for an additional 3 h. The cultures were then assayed for β-Gal activity, which is presented in Miller units as the mean ± σ^{(n − 1)} of results from at least three experiments.