Skip to main content
. 2011 Dec;193(23):6425–6435. doi: 10.1128/JB.06003-11

Fig. 5.

Fig. 5.

Reaction requirements of the RuvBFH-catalyzed DNA helicase reaction. (A) ATP dependence of the DNA helicase activity of RuvBFH. Reactions with substrate II and 2.7 μM RuvBFH were performed in the absence (lane 1) or presence of ATP at a concentration of either 0.25, 0.5, 1, 2, or 5 mM (as indicated above the lanes). (B) Nucleotide cofactor dependence of the DNA helicase activity of RuvBFH. Reactions with substrate II and 2.7 μM RuvBFH were performed in the absence (lane 1) or presence of a 2 mM concentration of either ATP (lane 2), ATPγS (lane 3), dATP (lane 4), dCTP (lane 5), dGTP (lane 6), or dTTP (lane 7). (C) Mg2+ dependence of the DNA helicase activity of RuvBFH. Reactions with substrate II and 2.7 μM RuvBFH were performed in the absence (lane 1) or presence of various concentrations of Mg2+ (as indicated above the lanes). (D) Reactions with substrate II and 2.7 μM RuvBFH were performed in the absence (lane 1) or presence of various concentrations of Mn2+ (as indicated above the lanes). The labeling of substrate and reaction products is similar to that used in Fig. 3.