Fig. 6.
DNA helicase activities of MBP fusion proteins of RuvBFH, RuvBM129, and RuvBMge. (A) DNA helicase activities of MBP-RuvBFH, MBP-RuvBM129, and MBP-RuvBMge. Reactions were performed with substrate II and various concentrations of MBP-β-galactosidase-α (MBP; lanes 2 to 4), MBP-RuvBFH (lanes 6 to 8), MBP-RuvBM129 (lanes 10 to 12), and MBP-RuvBMge (lanes 14 to 16). Each protein was tested at a concentration of 0.07, 0.22, and 0.66 μM (as indicated by the triangles above the lanes). (B) The percentage of unwinding (the percentage of displaced oligonucleotide) of substrate II by MBP-RuvBFH (■), MBP-RuvBMge (□), MBP-RuvBM129 (▴), and MBP (○). The displaced oligonucleotides were measured from the gel shown in panel A as the percentage of released product relative to the total substrate in the reaction mixture.