Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristics | Reference or source |
|---|---|---|
| E. coli XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F′ proAB lacIqZΔM15 Tn10 (Tcr)] | Stratagene |
| A. tumefaciens strains | ||
| A348 | C58 background carrying pTiA6 | 13 |
| AB510 | A348, gguA deletion | This study |
| AB520 | A348, gguB deletion | This study |
| AB530 | A348, gguC deletion | This study |
| AB540 | A348, gguAB deletion | This study |
| AB533 | A348, but with RGS-6His-tagged gguC | This study |
| AB600 | A348, with gene locus Atu1113 deletion | This study |
| AB601 | A348 but with RGS-6His tag at the N terminus of the Atu1113-encoded protein | This study |
| AB511 | A348, gguA and gbpR deletion | This study |
| Plasmids | ||
| pYW15b | Broad-host-range expression vector, PN25-MCS-STOP, IncW, and pBR322ori, Ampr | 30 |
| pBBR1MCS-5 | Broad-host-range plasmid cloning vector; Gmr | 18 |
| pK18mobsacB | Suicide plasmid in Xanthomonas campestris pv. campestris; Kmr | 27 |
| pJZ10 | gguA deletion plasmid in pK18mobsacB | This study |
| pJZ11 | gguB deletion plasmid in pK18mobsacB | This study |
| pJZ12 | gguC deletion plasmid in pK18mobsacB | This study |
| pJZ13 | gguAB deletion plasmid in pK18mobsacB | This study |
| pJZ14 | Gene locus Atu1113 deletion plasmid in pK18mobsacB | This study |
| pJZ15 | RGS-6His-tagged gguC recombination plasmid in pK18mobsacB | This study |
| pJZ16 | PN25-gguC in pYW15b | This study |
| pJZ17 | PN25-Atu1113 in pYW15b | This study |
| pJZ18 | pBBR1MCS-5+P2+gguA | This study |
| pJZ19 | pBBR1MCS-5+P2+gguB | This study |
| pJZ20 | pBBR1MCS-5+P2+gguA-His | This study |
| pJZ21 | pBBR1MCS-5+P2+gguA(K44A)-His | This study |
| pJZ22 | RGS-6His-tagged gene locus Atu1113 recombination plasmid in pK18mobsacB | This study |