Fig. 5.

Second-site mutations in the ΔsagS strain implicate three SagS-dependent phenotypes as HptB dependent, linking SagS to the GacS/GacA/RsmZ regulatory pathway. (A) Evaluation of attachment to polystyrene by crystal violet staining 1 and 24 h after initial attachment in the ΔsagS strain harboring various second-site mutations, relative to that in P. aeruginosa PAO1. (B) rsmAYZ RNA levels in various ΔsagS strains grown planktonically, relative to those of PAO1, as determined by qRT-PCR. (C) Biofilm architecture of the ΔsagS strain harboring various second-site mutations. Biofilms were visualized by CSLM after 144 h. White bars = 100 μm.