Fig. 2.

Effect of manipulating the c-di-GMP pool on hapA, hapR, and rpoS mRNA expression. Strain C7258, containing plasmids expressing VieA, VieA(E170A), and VCA0956, was grown in TSB medium, and PDE or DGC expression was induced as described in Materials and Methods. Total RNA was extracted, and hapA, hapR, and rpoS mRNAs were measured by qRT-PCR. Each value represents the mean for three independent cultures. Error bars indicate standard deviations (*, P < 0.05; **, P < 0.01 for comparison of induced versus control cultures).