Table 1.
V. cholerae strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| C7258 | Wild type O1; El Tor biotype | Peru, 1991 |
| N16961 | Wild type O1; El Tor biotype; hapR negative | Bangladesh, 1975 |
| AJB50 | C7258 ΔrpoS | 30 |
| AJB51 | C7258 ΔhapR | 30 |
| AJB52 | C7258 ΔhapRΔrpoS | This study |
| AJB501 | C7258 ΔluxO | This study |
| AJB502 | C7258 rpoS::pCVDRpoS-FLAG | This study |
| AJB503 | AJB51 rpoS::pCVDRpoS-FLAG | This study |
| AJB504 | AJB501 rpoS::pCVDRpoS-FLAG | This study |
| N16961-FLAG | N16961 rpoS::pCVDRpoS-FLAG | This study |
| SZS007 | C7258 luxC-lacZ reporter | 47 |
| SZS012 | SZS007 ΔluxO | 47 |
| HX01 | SZS007 hapRp* | This study |
| HX02 | SZS007 ΔvpsT | This study |
| HX03 | AJB51 ΔvpsT | This study |
| AJB505 | SZS007 ΔvqmA | This study |
| Plasmids | ||
| pAT1568 | PDE VieA in pBAD33 | 51 |
| pAT1615 | Inactive VieA(E170A) in pBAD33 | 51 |
| pAT1662 | DGC VCA0956 in pBAD33 | 51 |
| pCMW75 | DGC QrgB expression vector | 54 |
| pTT3 | rrnB T1T2 transcription terminator in pUC19 | 44 |
| pCVDΔRpoS2 | DNA fragment carrying rpoS deletion in pCVD442 | 30 |
| pUCΔvqmA | SacI-BamHI and BamHI-SphI DNA fragments flanking vqmA sequentially cloned in pUC19 | This study |
| pCVDΔvqmA | SacI-SphI fragment harboring vqmA deletion in pCVD442 | This study |
| pUCΔvpsT | SacI-BamHI and BamHI-SphI DNA fragments flanking vpsT sequentially cloned in pUC19 | This study |
| pΔvpSTKm | 1.2-kb BamHI fragment encoding a Km resistance cassette from pUC4Ka in pUCΔvpsT | This study |
| pCVDΔvpsTKm | SacI-SphI fragment from pΔvpsTKm in pCVD442 | This study |
| pTT3RpoS-FLAG | SphI-PstI fragment containing rpoS ORF in pTT3 | This study |
| pCVDRpoS-FLAG | SphI-XbaI rpoS-FLAG and rrnB T1T2 fragment in pCVD442 | This study |
| pCVDhapRp* | Mutant hapR promoter in pCVD442 | This study |
a
The GenBank accession number of pUC4K is X06404.1.