Table 2.
Oligonucleotide primers
| Primer | Sequence (5′→3′)a |
|---|---|
| HapA1010 | GCACGGCGTTCAGTTATGCTTGTA |
| HapA1551 | AGGTAAAACGCGCGGTTAAACACG |
| HapR-F1 | GCCTCTAGATGAATTTGACGAGCAAGATC |
| HapR-R1 | GATGCATGCCTCAAACCAGACTTTGAG |
| HapRmut-F | CTTTAAGTAGCAAATAGCAAAATAATCATTAG |
| HapRmut-R | CTAATGATTATTTTGCTATTTGCTACTTAAAG |
| HapR589 | CGCCTCAAAAACGCAAACTACAACT |
| HapR1046 | ATGGAGTAGAAGATGCCGTGGAAC |
| RecA578 | GTGCTGTGGATGTCATCGTTGTTG |
| RecA863 | CCACCACTTCTTCGCCTTCTTTGA |
| RpoS3 | GAAGCATGCCAATACCGTAACCAAA |
| RpoS13 | GCCGAATTCGAGTGTCAGCAATAC |
| RpoS576 | CGATTTTGAAGATGAAGCACTGGAAG |
| RpoS994 | GCCAGATCTGTATTCGACGTTAAAC |
| RpoS1003 | TGTTTGGTTCATCAGCGCACGTTC |
| RpoS1020 | CTTCTGCAGTTACTTGTCGTCATCG |
| RpoSP189 | GGCTAGCAAAGGAAGGACAAACTGT |
| VpsT56 | CCAGATTGTTGAAAGAGGCGTTAG |
| VpsT252 | TGCGGACAGTTTATGATGACCTCT |
| VpsT690 | CTGCAAACACATATCGAGCTCTTCT |
| VpsT1220 | GCAGGATCCAAGCATTCTAACGTTTA |
| VpsT1395 | GAAGGATCCTGATGATGTTTTGACC |
| VpsT2093 | GGGATTATGAAACGTTTGGAAAAGCC |
| VqmA645 | GTAGAGCTCCATTCGGGTTAACTTG |
| VqmA1519 | GAAGGATCCGAACACACAGAGGTAT |
| VqmA2455 | GAAGGATCCCAGTAAGCAACAACGT |
| VqmA3334 | CAAGCATGCATGAGTGGTTCCTGTT |
a
Restriction sites used for directional cloning are underlined.