Table 3.
Effect of PDE VieA and DGC VCA0956 induction on c-di-GMP content
| Strain | c-di-GMP content (pg/mg of wet wt)a |
|---|---|
| C7258 | 3.0 ± 0.6 |
| C7258 ΔhapR (AJB51) | 11.2 ± 2.3 |
| C7258 ΔrpoS (AJB50) | 10.8 ± 0.7 |
| C7258 ΔhapR ΔrpoS (AJB52) | 22.7 ± 1.9 |
| C7258 + VieA control | 2.6 ± 0.2 |
| C7258 + VieA induced | 0.7 ± 0.1 |
| C7258 + VCA0956 control | 2.9 ± 0.8 |
| C7258 + VCA0956 induced | 180.7 ± 9.7 |
a
Cultures were grown to an OD600 of 2.0 in TSB medium induced with l-arabinose as required; c-di-GMP content was measured after a 4-h incubation period.