Fig. 9.

In vivo coimmunoprecipitation for SecA2 and Gap1 from the gap3 mutant. Cell lysates of the wild-type strain with pVPT-Gap1 (WT/pVPT-Gap1) or pVT1666 (WT/pVT1666) and cell lysates of the gap3 mutant with pVPT-Gap1 (gap3/pVPT-Gap1) or pVT1666 (gap3/pVT1666) were subjected to immunoprecipitation with monoclonal GFP antibody and then analyzed by Western blotting using polyclonal anti-SecA2 (A). The expression of SecA2 and GFP fusion proteins was detected by Western blot analyses using anti-SecA2 (B) and anti-GFP (C) antibodies, respectively.