Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant properties | Source or reference |
|---|---|---|
| Strains | ||
| E. coli Top10 | For propagation of the recombinant plasmids | Invitrogen |
| S. parasanguinis FW213 | Wild type | 10 |
| S. parasanguinis fap1 mutant | Wild type; fap1::aphA3; Kanr | 32 |
| S. parasanguinis gap1 mutant | Wild type; gap1::aphA3; Kanr | 18 |
| S. parasanguinis gap3 mutant | Wild type; gap3::aphA3; Kanr | 18 |
| S. parasanguinis secA2 mutant | Wild type; secA2::Tn5; Kanr | 18 |
| S. agalactiae J48 | Wild type | 23 |
| S. agalactiae 2603V/R | Wild type | 27 |
| S. sanguinis SK36 | Wild type | 34 |
| S. pneumoniae TIGR4 | Wild type | 28 |
| Plasmids | ||
| pGEX-5X-1 | GST fusion protein expression vector; Ampr | Amersham |
| pGEX-Gap1 | gap1 from FW213 cloned in pGEX-5X-1; Ampr | This study |
| pGEX-Asp1 (J48) | asp1 from J48 cloned in pGEX-5X-1; Ampr | This study |
| pGEX-Gap3 | gap3 from FW213 cloned in pGEX-5X-1; Ampr | 18 |
| pGEX-Asp3 (J48) | asp3 from J48 cloned in pGEX-5X-1; Ampr | This study |
| pGEX-SecA2 | secA2 from FW213 cloned in pGEX-5X-1; Ampr | This study |
| pGEX-SecA2 (J48) | secA2 from J48 cloned in pGEX-5X-1; Ampr | This study |
| pGEX-SecA | secA from FW213 cloned in pGEX-6P-1; Ampr | This study |
| pGBKT7 | In vitro translation vector; Kanr | Clontech |
| pBD-Gap1 | gap1 from FW213 cloned in pGBKT7; Kanr | 18 |
| pBD-Asp1 (J48) | asp1 from J48 cloned in pGBKT7; Kanr | This study |
| pBD-Gap3 | gap3 from FW213 cloned in pGBKT7; Kanr | 18 |
| pBD-Asp3 (J48) | asp3 from J48 cloned in pGBKT7; Kanr | This study |
| pBD-SecA2 | secA2 from FW213 cloned in pGBKT7; Kanr | This study |
| pBD-SecA2 (J48) | secA2 from J48 cloned in pGBKT7; Kanr | This study |
| pBD-SecA | secA from FW213 cloned in pGBKT7; Kanr | This study |
| pVT1666 | E. coli-Streptococcus shuttle vector; Ermr | 8 |
| pVPT-Gap1 | gap1 from FW213 cloned in pVT1666; Ermr | 36 |
| pVPT-Asp1 (J48) | asp1 from J48 cloned in pVT1666; Ermr | This study |
| pVPT-Asp1 (2603) | asp1 from 2603V/R cloned in pVT1666; Ermr | This study |
| pVPT-Asp1 (SK36) | asp1 from SK36 cloned in pVT1666; Ermr | This study |
| pVPT-Asp1 (TIGR4) | asp1 from TIGR4 cloned in pVT1666; Ermr | This study |
| pVPT-Gap3 | gap3 from FW213 cloned in pVT1666; Ermr | 20 |