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. 2011 Dec;193(23):6560–6566. doi: 10.1128/JB.05668-11

Table 2.

Primers used in this study

Primer Sequence
Gap1-EcoRI-1F GATCAGAATTCATGTTTTATTTTGTACCTTC
Gap1-XhoI-1575R GATCTCTCGAGTTTCTTTTTTAGCATACCTTTC
SecA2-BamHI-F CGGGATCCATATGGAAAAAATAAAACAAAAG
SecA2-BamHI-R CGGGATCCTACAAAATAAATTGATAAACC
SecA2-PstI-R AACTGCAGTACAAAATAAATTGATAAACC
SecA-SmaI-F GATCACCCGGGTATGGCAAATTTATTAAAAAC
SecA-XhoI-R GATCACTCGAGTCAAAATTTACGTCCGTGAC
SecA-NdeI-F GATCACATATGGCAAATTTATTAAAAAC
SecA-PstI-R GATCACTGCAGTCAAAATTTACGTCCGTGAC
Asp1-J48-BamHI-F GATCAGGATCCCCATGTTTTATTTTATTCCTTCG
Asp1-J48-BamHI-R GATCAGGATCCTTATTCTTTTTCTAATAATTTTCG
Asp3-J48-BamHI-F GATCAGGATCCCCATGATTTTGGGAGAGTGTTTAG
Asp3-J48-BamHI-R GATCAGGATCCTCATTTTTTATCCTTAGAAAATG
SecA2-J48-BamHI-F GATCAGGATCCCCATGAAAAAAGTTGTTGTTAAACC
SecA2-J48-BamHI-R GATCAGGATCCTTATACATAATATATTGAGTAACTTC
Asp1-J48-SalI-F GATCAGTCGACATGTTTTATTTTATTCCTTCG
Asp1-2603-SalI-F GATCAGTCGACATGTTTCATTTTATTCCATC
Asp1-2603-KpnI-R GATCAGGTACCATAACTATTTATAATTCTTTC
Asp1-SK36-SalI-F GATCAGTCGACATGTATTACTTTATTCCTTC
Asp1-SK36-BamHI-R GATCAGGATCCTTATTTTGCATTCTCAGC
Asp1-TIGR4-SalI-F GATCAGTCGACATGTATTATTTTATTCCAG
Asp1-TIGR4-BamHI-R GATCAGGATCCTTAAGTTCCTTTGACATTTTTAAC