Fig. 4.
Size exclusion chromatograms of recombinant CT296 at various concentrations. The molecular mass of monomeric CT296 is 17.9 kDa. The molecular mass of a CT296 dimer would be 35.8 kDa. The components of a molecular mass ladder are marked at their respective elution volumes on a line above the chromatograms. (A) At a concentration of 1,000 μM, CT296 is predominantly present in a peak that approximately corresponds to the molecular mass of a dimer. Other, even-higher-order, structures can also be seen, in lower abundance. (B) At a concentration of 100 μM, CT296 elutes within fractions that correspond to the molecular mass of a monomer. (C) At a concentration of 10 μM, CT296 elutes as a peak that corresponds to the molecular mass of a monomer.