Fig. 1.

OspA-CaM fusion constructs. Cartoons of OspA-CaM fusion constructs generated and used in this study show triacyl anchor lipids in black, OspA peptides in green (N-terminal tether and β-strands) and orange (C-terminal α-helix), linker peptides in blue, and CaM peptides in red. Tether and linker peptide sequences are given in one-letter amino acid code. Mutant nomenclature follows that of earlier publications (50, 51). Briefly, modified residues are numbered according to their prolipoprotein positions; numbers in lipoprotein tether-CaM fusion constructs indicate the C-terminal tether residue present in the fusion.