Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Borrelia burgdorferi strains | ||
| B313 | Clone of B31 ATCC 35210 (cp26, cp32-1, cp32-2/7, cp32-3, lp17); OspA− | 46 |
| B31-e2 | Clone of B31 ATCC 35210 (cp26, cp32-1, cp32-3, cp32-4, lp17, lp38, lp54); OspA+ | 2 |
| Plasmids | ||
| pBSXcam | pBS vector containing the Xenopus laevis cam coding sequence (NCBI BC072232) | Gift from G. Carlson |
| pCRW53 | pBSVΦ(ospAp-gfp) derivative with gfp gene under Post promoter control | 14, 57 |
| pCRW56 | pCRW53 lacking Kanr cassette NdeI site and two Tetr cassette NdeI sites, with NdeI site introduced 5′ of gfp gene | This study |
| pRJS1009 | pBSV2::PflaBOspA28-mRFP1 | 51 |
| pRJS1014 | pBSV2::PflaBOspA19-mRFP1 | 51 |
| pRJS1089 | pBSV2::PflaBOspA28ΔV21-mRFPΔ4 | 50 |
| pSC0999 | pBSV2::PflaBospA-His, lacking pBSV2 BamHI/XmaI MCSa | 50 |
| pSC2001 | pBSV2::PflaBOspA-CaM | This study |
| pSC2002 | pBSV2::PflaBOspA28-CaM | This study |
| pSC2003 | pBSV2::PflaBOspA19-CaM | This study |
| pSC2004 | pBSV2::PflaBOspA28ΔV21-CaM | This study |
| pSC2005 | pCRW56::Post OspA19-Xcam | This study |
| pSC2006 | pCRW56::Post OspA28-ΔV21-CaM | This study |
a
MCS, multiple-cloning site.