Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| DH5αF′ | Host for E. coli cloning; F′ 80dlacZΔM15 recA endA1 gyrA96 thi-1 hsdR17(rK− mK−) supE44 relA1 deoR Δ(lacZYA-argF)U169 | 15 |
| JM109 | Host for E. coli cloning | 52 |
| S17-1 | For conjugational transfer of plasmids; recA thi pro hsdRM+ RP4-2-Tc::Mu-Km::Tn7 | 42 |
| S17-1λpir | For conjugational transfer of plasmids | 42 |
| 2.4.3 | Wild-type strain of R. sphaeroides | ATCC 17025 |
| 2.4.1 | Wild-type strain of R. sphaeroides | Type strain |
| 2.4.9 | Wild-type strain of R. sphaeroides | ATCC 17029 |
| prrA strain | prrA::aph, 2.4.3 derivative; Kmr | 24 |
| fnrL strain | 2.4.3 with insertional inactivation of fnrL | 18 |
| ΔccoN strain | 2.4.3 with ΩSm/Sp in ccoN; deletion mutant | 16 |
| nap-β strain | 2.4.3 with insertional inactivation of napA-β; Kmr | This study |
| nap-α strain | 2.4.3 with insertional inactivation of napA-α; Tcr | This study |
| nap-α nap-β strain | 2.4.3 with inactivation of both nap-α and nap-β; Kmr Tcr | This study |
| fnrX strain | 2.4.3 with insertional inactivation of gene 3486; Tcr | This study |
| qsrR strain | 2.4.3 with insertional inactivation of gene 0408; Tcr | This study |
| Plasmids | ||
| pUC19 | Used for cloning in E. coli DH5α (Apr) | 52 |
| pRK415 | Broad-host-range plasmid (Tcr) | 19 |
| pSUP202-1 | Mobilizable suicide vector (Tcr Apr Cmr) | 42 |
| pKOK-6 | Source of lacZ-Km cassette (Tcr Kmr Apr) | 21 |
| pJP5603 | Mobilizable suicide vector (Kmr) | 33 |
| pNAP-βZ | pRK415 with the nap-β promoter fused to lacZ, transcriptional reporter (Tcr Kmr) | This study |
| pNAP-αZ | pRK415 with the nap-α promoter fused to lacZ, transcriptional reporter (Tcr Kmr) | This study |
| pNAP-βKO | pJP5603 with napA internal region (nap-β) (Kmr) | This study |
| pNAP-αKO | pSUP202-1 with napA internal region (nap-α) (Tcr) | This study |
| pFNRXKO | pSUP202-1 with fnrX internal region (Tcr) | This study |
| pQSRRKO | pSUP202-1 with qsrR internal region (Tcr) | This study |