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. 2011 Dec;193(23):6443–6451. doi: 10.1128/JB.00425-11

Fig. 1.

Fig. 1.

MltE in combination with MltC affects the expression of the rdar morphotype through CsgD. (A) Wild-type S. Typhimurium UMR1 and mutants of lytic transglycosylases MltE and MltC grown on Congo red agar plates at 28°C for 24 h. The mltE and mltC single mutants do not show any difference in rdar morphotype expression compared to that of wild-type UMR1, while the mltE mltC double mutant shows a visible downregulation of rdar morphotype expression. (B) MltE overexpression enhanced the rdar morphotype expression of UMR1 compared to that of the strain containing the vector control. Spot colonies were grown on Congo red agar plates at 28°C for 7 days. VC, vector control pBAD30. (C) CsgD and CsgA expression in S. Typhimurium UMR1 and its mltE and mltC mutants. Compared to wild-type UMR1, a significant reduction of CsgD and CsgA expression is observed in the mltE mltC double mutant. No change in CsgD and CsgA levels is detected in the mltE and mltC single mutants. The strains UMR1 ΔcsgD and UMR1 ΔcsgA were used as negative controls (n.c.) for CsgD and CsgA detection, respectively. Strains were grown on LB without NaCl agar at 28°C for 17 h.