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OmpT is regulated by Fur at the mRNA level. Dot blot analysis of equal amounts of RNA harvested from wild-type (N16961) and fur mutant (ARM713) cells grown in EZ-RDM with (+) or without (−) 5 μM FeSO4. RNA samples were probed with an ompT probe (upper panel), and a duplicate blot was probed with an rrsA probe (lower panel) to ensure equal amounts of RNA were present in the samples.
