Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| TOP10 | Cloning strain | Invitrogen |
| S17-1 | recA endA thi hsdR RP4-2 Tc::Mu-Kn::Tn7 Tpr Smr | 38 |
| R. sphaeroides | ||
| WS8-N | Wild type, spontaneous Nalr | 40 |
| RS1Y5 | WS8-N derivative, cheY5::aadA | This study |
| RS1B | WS8-N derivative, mcpB::aadA | This study |
| RS1S | WS8-N derivative, tlpS::aadA | This study |
| RS1A | WS8-N derivative, mcpA::aadA | This study |
| RS1D | WS8-N derivative, cheD::aadA | This study |
| RS1Y1 | WS8-N derivative, cheY1::aadA | This study |
| RS1A1 | WS8-N derivative, cheA1::aadA | This study |
| RS1W1 | WS8-N derivative, cheW1::aadA | This study |
| RS1R1 | WS8-N derivative, cheR1::aadA | This study |
| RS1Y2 | WS8-N derivative, cheY2::aadA | This study |
| RS12432 | WS8-N derivative, RSP2432::aadA | This study |
| AM1 | SP13 derivative, fla2+ | 11 |
| RS2Y5 | AM1 derivative, cheY5::aadA | 11 |
| RS2B | AM1 derivative, mcpB::aadA | This study |
| RS2S | AM1 derivative, tlpS::aadA | This study |
| RS2A | AM1 derivative, mcpA::aadA | This study |
| RS2D | AM1 derivative, cheD::aadA | This study |
| RS2Y1 | AM1 derivative, cheY1::aadA | 11 |
| RS2A1 | AM1 derivative, cheA1::aadA | This study |
| RS2W1 | AM1 derivative, cheW1::aadA | This study |
| RS2R1 | AM1 derivative, cheR1::aadA | This study |
| RS2Y2 | AM1 derivative, cheY2::aadA | 11 |
| RS22432 | AM1 derivative, RSP2432::aadA | This study |
| Plasmids | ||
| pTZ19R | Cloning vector; pUC derivative; Apr | Pharmacia |
| pJQ200mp18 | Suicide vector used for gene replacement; Gmr | 34 |
| pRK415 | pRK404 derivative, used for expression in R. sphaeroides; Tcr | 19 |
| pRKtlpS | pRK415 derivative expressing tlpS | This study |
| pRKmcpA | pRK415 derivative expressing mcpA | This study |
| pRKcheD | pRK415 derivative expressing cheD | This study |
| pRKcheA1 | pRK415 derivative expressing cheA1 | This study |
| pRKcheW1 | pRK415 derivative expressing cheW1 | This study |
| pRKcheR1 | pRK415 derivative expressing cheR1 | This study |
| pRK2432 | pRK415 derivative expressing RSP2432 | This study |