Fig. 7.

Deletion of hopanoid biosynthesis genes does not affect intramacrophage replication or survival of B. cenocepacia K56-2. RAW 264.7 macrophages were infected with the MH1K, ΔBCAM2828, or Δshc strain. The infected cells were lysed at 0, 24, and 48 h postinfection, and intracellular bacteria were enumerated by serial dilution and colony counts on LB agar plates. The results represent the averages and standard errors of three independent experiments. No significant differences in replication or survival were found using an unpaired t test.