Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| B. cenocepacia | ||
| MH1K | ET12 clone related to J2315; CF clinical isolate; made Gms via deletion of an efflux pump | 20 |
| MH1K ΔBCAM2828 | Deletion of BCAM2828 in MH1K | This study |
| MH1K ΔBCAM2831 | Deletion of BCAM2831 in MH1K | This study |
| MH1K ΔBCAS0167 | Deletion of BCAS0167 in MH1K | This study |
| MH1K Δshc | Deletion of BCAM2831 and BCAS0167 in MH1K | This study |
| E. coli | ||
| GT115 | F−mcrA Δ(mrr-hsdRMSmcrBC) φ80′lacZΔM15 ΔlacX74 recA1 rpsL (StrA) endA1 Δdcm uidA(ΔMluI)::pir-116 ΔsbcC-sbcD | Laboratory strain |
| DH5α | λ− φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | Laboratory strain |
| Plasmids | ||
| pBCAM2828 | BCAM2828 in pSCrhaB2 | This study |
| pBCAM2831 | BCAM2831 in pSCrhaB2 | This study |
| pBCAS0167 | BCAS0167 in pSCrhaB2 | This study |
| pDAI-SceI-SacB | oripBBR1 TetrPdhfr mob+; expressing I-SceI; also expresses negative selection marker SacB | 20 |
| pDelBCAM2828 | pGPI-SceI with fragments flanking BCAM2828 | This study |
| pDelBCAM2831 | pGPI-SceI with fragments flanking BCAM2831 | This study |
| pDelBCAS0167 | pGPI-SceI with fragments flanking BCAS0167 | This study |
| pGPI-SceI | oriR6K ΩTprmob+, including an I-SceI restriction site | 13 |
| pRK2013 | oricolE1; RK2 derivative; Kanrmob+tra+ | 67 |
| pSCrhaB2 | oripBBR1rhaR rhaS PrhaB Tprmob+ | 6 |
| pShc | BCAM2831 and BCAS0167 in pSCrhaB2 | This study |
a
Tpr, trimethoprim resistance; Kanr, kanamycin resistance; Tetr, tetracycline resistance; Gms, gentamicin sensitive.