Table 2.
Primers used in this study
| Primer no. | Primer sequence (5′-3′)a | Restriction enzyme |
|---|---|---|
| 4820 | GTCGATGGTACCCTTCGGCAGCCTGTGGCTGTCGCATCAT | KpnI |
| 4821 | GTCGCATCTAGAAGCACGAGATACGGGCCGGACGGCATG | XbaI |
| 4822 | ATCGATAATATTGAACTCGCCGCGCCCGACACGTTCAGCGC | SspI |
| 4823 | GATCGAGGTACCGACGAGCAGCACGTATTCGGCGGGAATCG | KpnI |
| 4824 | GTCGATGGTACCCAGCAGAACGAAGAAGGCCTGTGGGACGA | KpnI |
| 4825 | ATCGATAATATTCAGCACGTCGGTACTCGCGGCCGGATTGG | SspI |
| 4826 | GATCGAGGTACCTCCATGAAATGCATCATCAGGATGTATTC | KpnI |
| 4827 | GTCGATGGTACCGACGGCTTCTGGTGGCACCGCTCGCACAA | KpnI |
| 4828 | GTCGCATCTAGAAAGCCGCGATGTGCTGCATCGTGCCGCGC | XbaI |
| 4829 | GTCGCATCTAGAGGATCGACGATCGCGCGGCACACCGCGAA | XbaI |
| 4858 | ATCGATAATATTGACGATATCGACCGGCGCGCTCTGCGCGAA | SspI |
| 4859 | GATCGAGGTACCCGTAGACGCCGCTGAAGGCGGCGATGACGA | KpnI |
| 4987 | GTACAGCTCCTGCTGCTGTTGCGGCGTG | None |
| 4988 | GCGGCCTGCTTCAGCAGGTCGGTCGCGC | None |
| 4989 | ATTCGCGGCTCGACGCGTTCGAGTTCGG | None |
| 4992 | CGGCTGCTTCAGCAGTTCGCGCAGGATC | None |
| 5097 | CAGCTTCCGAAAGGCCATG | None |
| 5098 | ATCTGCTGCCCGAACTCGTC | None |
| 5268 | ATATATCATATGGCGGACGTGCTCCAGACGCAATAAAGAG | NdeI |
| 5269 | ATATATTCTAGAGCCACGCGTTACATCCCGACCGTCACG | XbaI |
| 5270 | ATATATCATATGACTGCACGTCTCGAGATAAACATTCCG | NdeI |
| 5271 | ATATATTCTAGACCCGGCGCGCGACCGCTCAGGCGAGC | XbaI |
| 5413 | ATATATCATATGGTCGATTATGTAAATATCCCCATACAAATG | NdeI |
| 5414 | ATATATTCTAGAGATTCGCACCTTATTCATTGATTCCTTGCGA | XbaI |
| 5415 | ATATATTCTAGAAGCACTGCACGTCTCGAGATAAACATTCCG | XbaI |
| 5416 | ATATATAAGCTTGCACCCCGGCGCGCGACCGCTCAGGCGAGC | HindIII |
a
Incorporated restriction enzyme recognition sites are underlined in the primer sequences.