Skip to main content
. 2011 Dec;193(23):6674–6682. doi: 10.1128/JB.05714-11

Fig. 8.

Fig. 8.

Electrophoretic mobility shift assay. (A and B) Target DNA, X. oryzae pv. oryzae hrpG promoter region (A) or X. oryzae pv. oryzae hrpG kdgR box deletion promoter region (B), was mixed with purified His-KdgRxoo protein. (C and D) Target DNA, X. axonopodis pv. citri hrpG promoter region (C) or X. oryzae pv. oryzae hrpG promoter region (D), was mixed with purified His-KdgRxac protein. The probe labeled with rhodamine dye (20 ng) was incubated in the absence (lane 1) or presence (lanes 2 to 5) of increasing amounts of the KdgR protein (50, 150, 300, and 500 nM).