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. 2011 Dec;31(23):4814–4831. doi: 10.1128/MCB.05819-11

Fig. 3.

Fig. 3.

Isolation of Ssu− substitutions in eIF1. (A) Summary of genetic selection used to isolate Ssu− alleles of SUI1 as suppressors of the recessive lethality of SUI5. The relevant genotype, the expression levels of his4-301 and PGAL-TIF5 (ON or OFF), and the growth phenotypes on medium containing galactose or glucose as carbon source are indicated for the parental strain (two rectangles on the left) and for Ssu− sui1 mutants (two rectangles on the right). See the text for further details. (B) Ssu− phenotypes of derivatives of a sui1Δ his4-301 PGAL-TIF5 strain with episomal SUI5 (PMY04) harboring the indicated SUI1 alleles. Tenfold serial dilutions of these strains plus strains PMY89 and PMY91 containing episomal TIF5 (p4119) or empty vector, versus SUI5 (last two rows) were spotted on SGal+His and SGal-His (containing 0.003 mM His). (C) In the top panel, the same strains as in panel B were streaked on SC containing 2% galactose, 1% raffinose as a carbon source, lacking Leu and tryptophan (Trp), and supplemented with 0.3 mM His (SGal+His). In the bottom panel, strains were streaked on SC lacking Leu and Trp and supplemented with 5-FOA (SC + 5-FOA). (D) Phenotypes of SUI1 Ssu− mutations in the absence of SUI5. Derivatives of sui1Δ his4-301 strain JCY03 containing the indicated SUI1 alleles were analyzed as in Fig. 1A.