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. 2011 Dec;31(23):4814–4831. doi: 10.1128/MCB.05819-11

Fig. 5.

Fig. 5.

SUI1 Ssu− mutations reduce SUI1 expression by exacerbating poor context. (A to C) SUI1 Ssu− mutations reduce eIF1 expression dependent on SUI1+ AUG context. Derivatives of JCY03 containing the indicated SUI1 alleles (A) or SUI1-opt alleles (B) were subjected to Western analysis as in Fig. 1C. Two different amounts of each extract differing by a factor of 2 were loaded in successive lanes. (C) Ratios of mean eIF1/Gcd6 values for SUI1 versus SUI1-opt alleles were calculated as in Fig. 1E. (D) Derivatives of JCY03 harboring the indicated SUI1 alleles and the SUI1-lacZ or SUI1-opt-lacZ reporter were analyzed for β-galactosidase activities as in Fig. 1G. (E) SUI1-lacZ and SUI1-opt-lacZ expression levels were determined in derivatives of JCY03 containing sc SUI1, hc SUI, or hc SUI1-opt, as indicated, as in Fig. 1G. (F) Western analysis of eIF1 expression in JCY03 derivatives containing the indicated sc or hc SUI1 alleles, conducted as in Fig. 1C.