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. 2011 Dec;31(23):4814–4831. doi: 10.1128/MCB.05819-11

Fig. 6.

Fig. 6.

Sui− mutations in eIF2β and eIF1A suppress the poor AUG context at SUI1. (A) Derivatives of sui1Δ PGAL-SUI3 strain PMY02 containing plasmid-borne SUI3 (p4450) or SUI3-2 (p4280) and either sc SUI1+ or sc SUI1-opt were cultured continuously in SD supplemented with His and Ura (with repression of chromosomal PGAL-SUI3) and subjected to Western analysis of eIF1 expression, as in Fig. 1C. Two different amounts of each extract differing by a factor of 2 were loaded in successive lanes. (B) Analysis of SUI1-lacZ and SUI1-opt-lacZ expression in the SUI1+ strains from panel A conducted as in Fig. 1G. (C) Western analysis of eIF1 in derivatives of sui1Δ PGAL-TIF11 strain PMY03 containing plasmid-borne TIF11+ (pDSO9), tif11-SE1*,SE2*+F131(pAS23), or tif11-17-21 (p4552), and either sc SUI1+ or sc SUI1-opt, conducted as in panel A. (D) Ratios of mean eIF1/Gcd6 values for SUI1 versus SUI1-opt alleles calculated for strains analyzed in panels A and C. (E) Analysis of SUI1-lacZ and SUI1-opt-lacZ expression in transformants of the strains from the SUI1+ strains from panel C, as in Fig. 1G.