Table 2.
Yeast strains used in this study
| Strain | Genotypea | Source or reference |
|---|---|---|
| JCY03 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG p1200 (sc URA3SUI1) | 8 |
| PMY30 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pJCB101 (sc LEU2SUI1) | This study |
| PMY31 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB01 (sc LEU2sui1-K56E) | This study |
| PMY32 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB02 (sc LEU2sui1-K60E) | This study |
| PMY33 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB03 (sc LEU2sui1-L96P) | This study |
| PMY98 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB30 (hc LEU2sui1-L96P) | This study |
| PMY34 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB04 (sc LEU2sui1-T15A) | This study |
| PMY35 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB05 (sc LEU2sui1-E48V) | This study |
| PMY36 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB06 (sc LEU2sui1-L51F) | This study |
| PMY37 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB07 (sc LEU2sui1-D61G) | This study |
| PMY38 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB08 (sc LEU2sui1-Q84H) | This study |
| PMY39 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB09 (sc LEU2sui1-E48V,L51F) | This study |
| PMY40 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pCFB04 (hc LEU2SUI1) | This study |
| PMY41 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB10 (sc LEU2SUI1-opt) | This study |
| PMY42 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB12 (sc LEU2sui1-K56E-opt) | This study |
| PMY43 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB13 (sc LEU2sui1-K60E-opt) | This study |
| PMY44 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB14 (sc LEU2sui1-L96P-opt) | This study |
| PMY45 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB15 (sc LEU2sui1-T15A-opt) | This study |
| PMY46 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB16 (sc LEU2sui1-E48V-opt) | This study |
| PMY47 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB17 (sc LEU2sui1-L51F-opt) | This study |
| PMY48 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB18 (sc LEU2sui1-D61G-opt) | This study |
| PMY49 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB19 (sc LEU2sui1-Q84H-opt) | This study |
| PMY50 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB20 (sc LEU2sui1-E48V,L51F-opt) | This study |
| PMY51 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG pPMB11 (hc LEU2SUI1-opt) | This study |
| PMY01 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 p1200 (sc URA3SUI1) | This study |
| PMY02 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-SUI3 p1200 (sc URA3SUI1) | This study |
| PMY03 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF11 p1200 (sc URA3SUI1) | This study |
| PMY04 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 p1200 (sc URA3SUI1) p4281 (sc TRP1 TIF5-G31R) | This study |
| PMY52 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 p4281 (sc TRP1 TIF5-G31R) pJCB101 (sc LEU2SUI1) | This study |
| PMY53 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 p4281 (sc TRP1 TIF5-G31R) pPMB04 (sc LEU2sui1-T15A) | This study |
| PMY54 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 p4281 (sc TRP1 TIF5-G31R) pPMB05 (sc LEU2sui1-E48V) | This study |
| PMY55 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 p4281 (sc TRP1 TIF5-G31R) pPMB06 (sc LEU2sui1-L51F) | This study |
| PMY56 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 p4281 (sc TRP1 TIF5-G31R) pPMB07 (sc LEU2sui1-D61G) | This study |
| PMY57 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 p4281 (sc TRP, TIF5-G31R) pPMB08 (sc LEU2sui1-Q84H) | This study |
| PMY58 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(AUU) sui1Δ::hisG kanMX6:PGAL1-TIF5 p4281 (sc TRP1 TIF5-G31R) pPMB09 (sc LEU2sui1-E48V,L51F) | This study |
| PMY59 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 p4281 (sc TRP1 TIF5-G31R) pCFB04 (hc LEU2SUI1) | This study |
| JCY04 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG)-myc10::KanMX sui1Δ::hisG p1200 (sc URA3SUI1) | 33 |
| JCY806 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG)-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1 TIF5-G31R) pJCB101 (sc LEU2SUI1) | This study |
| JCY807 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG)-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1, TIF5-G31R) pPMB04 (sc LEU2sui1-T15A) | This study |
| JCY808 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG)-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1 TIF5-G31R) pPMB05 (sc LEU2sui1-E48V) | This study |
| PMY60 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG)-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1, TIF5-G31R) pPMB06 (sc LEU2sui1-L51F) | This study |
| JCY809 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG)-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1 TIF5-G31R) pPMB07 (sc LEU2sui1-D61G) | This study |
| JCY810 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG)-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1 TIF5-G31R) pPMB08 (sc LEU2sui1-Q84H) | This study |
| PMY61 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG)-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1 TIF5-G31R) pPMB09 (sc LEU2sui1-E48V,L51F) | This study |
| PMY62 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG)-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1 TIF5-G31R) pCFB04 (hc LEU2SUI1) | This study |
| H466 | MATαhis1-29 gcn2-101 gcn3-101 ino1 ura3-52 | A. Hinnebusch |
| PMY16 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 HIS4-myc10::KanMX sui1Δ::hisG p1200 (sc URA3SUI1) | This study |
| PMY63 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 HIS4-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1 TIF5-G31R) pJCB101 (sc LEU2SUI1) | This study |
| PMY64 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 HIS4-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1, TIF5-G31R) pPMB04 (sc LEU2sui1-T15A) | This study |
| PMY65 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 HIS4-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1 TIF5-G31R) pPMB05 (sc LEU2sui1-E48V) | This study |
| PMY66 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 HIS4-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1, TIF5-G31R) pPMB06 (sc LEU2sui1-L51F) | This study |
| PMY67 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 HIS4-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1 TIF5-G31R) pPMB07 (sc LEU2sui1-D61G) | This study |
| PMY68 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 HIS4-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1 TIF5-G31R) pPMB08 (sc LEU2sui1-Q84H) | This study |
| PMY69 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 HIS4-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1 TIF5-G31R) pPMB09 (sc LEU2sui1-E48V,L51F) | This study |
| PMY70 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 HIS4-myc10::KanMX sui1Δ::hisG p4281 (sc TRP1 TIF5-G31R) pCFB04 (hc LEU2SUI1) | This study |
| PMY71 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG p4280 (sc TRP1 SUI3-2) pJCB101(sc LEU2SUI1) | This study |
| PMY72 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG p4280 (sc TRP1 SUI3-2) pPMB04 (sc LEU2sui1-T15A) | This study |
| PMY73 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG p4280 (sc TRP1 SUI3-2) pPMB05 (sc LEU2sui1-E48V) | This study |
| PMY74 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG p4280 (sc TRP1 SUI3-2) pPMB06 (sc LEU2sui1-L51F) | This study |
| PMY75 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG p4280 (sc TRP1 SUI3-2) pPMB07 (sc LEU2sui1-D61G) | This study |
| PMY76 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG p4280 (sc TRP1 SUI3-2) pPMB08 (sc LEU2sui1-Q84H) | This study |
| PMY77 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG p4280 (sc TRP1 SUI3-2) pPMB09 (sc LEU2sui1-E48V,L51F) | This study |
| PMY78 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG p4280 (sc TRP1 SUI3-2) pCFB04 (hc LEU2SUI1) | This study |
| PMY79 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-SUI3 p4450 (sc TRP1 SUI3) pJCB101 (sc LEU2SUI1) | This study |
| PMY80 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-SUI3 p4450 (sc TRP1 SUI3) pPMB10 (sc LEU2SUI1-opt) | This study |
| PMY81 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-303(ACG) sui1Δ::hisG kanMX6:PGAL1-SUI3 p4280 (sc TRP1 SUI3-2) pJCB101 (sc LEU2SUI1) | This study |
| PMY82 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-SUI3 p4280 (sc TRP1 SUI3-2) pPMB10 (sc LEU2SUI1-opt) | This study |
| PMY83 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF11 pDSO9 (sc LEU2 TIF11) pPMB21 (sc TRP1SUI1) | This study |
| PMY84 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF11 pDSO9 (sc LEU2 TIF11) pPMB22 (sc TRP1SUI1-opt) | This study |
| PMY85 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF11 p4552 (sc LEU2TIF11-NDSDG17-21AAAAA) pPMB21 (sc TRP1SUI1) | This study |
| PMY86 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF11 p4552 (sc LEU2TIF11-NDSDG17-21AAAAA) pPMB22 (sc TRP1SUI1-opt) | This study |
| PMY87 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF11 pAS23 (sc LEU2TIF11-FGFESDE121-127AAAAAAA,FEFGN131-135FAAAA) pPMB21 (sc TRP1SUI1) | This study |
| PMY88 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF11 pAS23 (sc LEU2TIF11-FGFESDE121-127AAAAAAA,FEFGN131-135FAAAA) pPMB22 (sc TRP1SUI1-opt) | This study |
| PMY89 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 p4119 (sc TRP1 TIF5-FL) pJCB101 (sc LEU2SUI1) | This study |
| PMY90 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 p4119 (sc TRP1 TIF5-FL) pPMB10 (sc LEU2SUI1-opt) | This study |
| PMY91 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 YCplac22 (sc TRP1) pJCB101 (sc LEU2SUI1) | This study |
| PMY92 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 YCplac22 (sc TRP1) pPMB10 (sc LEU2SUI1-opt) | This study |
| PMY93 | MATaura3-52 leu2-3 leu2-112 trp1Δ-63 his4-301(ACG) sui1Δ::hisG kanMX6:PGAL1-TIF5 p4281 (sc TRP1 TIF5-G31R) pPMB10 (sc LEU2SUI1-opt) | This study |
a
hc, high copy number; sc, single copy.