Skip to main content
. 2011 Dec;31(23):4692–4705. doi: 10.1128/MCB.05979-11

Fig. 2.

Fig. 2.

Osteoclast progenitors reside in quiescent PPARγ+ bone marrow cells. (A) Schematic diagram of the in vivo marking of osteoclast stem/progenitor cells. (B) A pulse-chase strategy labels the quiescent PPARγ+ stem/progenitor cells as LRCs. (C) Lineage tracing of mature osteoclasts from PPARγ+ progenitors using an indelible LacZ reporter (left) and a chaseable GFP reporter (right). OcP, osteoclast progenitor/precursors; B, bone; BM, bone marrow. Scale bars, 25 μm. (D) Percentage of LRCs or GFP− cells that formed osteoclasts when plated at a single cell per well (n = 96). (E to G) Flow cytometry analyses of the LRC, GFP+, and GFP− bone marrow populations (n = 6). The error bars indicate SD. (E) Percentages of total GFP+ cells and LRCs in the bone marrow. (F) Percentage of cells expressing stem/progenitor cell markers (C-Kit, Sca-1, CD135, SiglecF, or Notch1), macrophage marker (CD11b), or erythrocyte marker (Ter119) in each population. For P values, the asterisks compare total GFP+ cells or LRCs with GFP− cells and the pluses compare LRCs with total GFP+ cells. (G) Percentage of cells expressing CD45, CD115 (M-CSFR; c-fms), or CD265 (RANK). *, P < 0.05; ++, P < 0.01; ***, P < 0.005; ****, P < 0.001; ***** or +++++, P < 0.0005; ****** or ++++++, P < 0.0001.