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. 2011 Dec;31(23):4692–4705. doi: 10.1128/MCB.05979-11

Fig. 3.

Fig. 3.

Notch activation in PPARγ-expressing cells causes high bone mass. (A) Schematic diagram of PTNICD mice. (B and C) μCT analysis of the tibiae from PTNICD or control mice (5 months old; male; n = 6). (B) Representative images of the trabecular bone of the tibial metaphysis (top; scale bar, 10 μm) and the entire proximal tibia (bottom; scale bar, 1 mm). Ctrl, control. (C) Quantification of trabecular bone volume and architecture. BS, bone surface; Tb.N, trabecular number; Tb.Sp, trabecular separation. The error bars indicate SD. (D) Urinary CTX-1 (normalized to urinary creatinine) (n = 6). (E) Serum osteocalcin (n = 6). (F and G) Bone histomorphometry (n = 6). (F) Representative images of TRAP-stained femoral sections. Scale bar, 100 μm. (G) Quantification of osteoclast surface (Oc.S/B.S) and number (Oc.N/B.Ar); B.Ar, bone area. (H) PTNICD mice exhibited extramedullary hematopoiesis in the spleen (n = 6). (I) Osteoclast precursor proliferation was decreased in the PTNICD cultures. (Left) Schematic diagram of the proliferation assay. (Right) Quantification of BrdU incorporation (n = 3). (J and K) Osteoclast differentiation was blunted in the PTNICD culture. (J) Representative images of TRAP-stained osteoclast differentiation cultures. Scale bar, 25 μm. (K) Representative osteoclast marker expression (n = 3). R, RANKL; V, vehicle; B, BRL. (L) NICD expression in control or PTNICD differentiation culture (n = 3). (M) Tibial RANKL/OPG mRNA ratio in control or PTNICD mice (n = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.005; ****, P < 0.001; *****, P < 0.0005; n.s., nonsignificant (P > 0.05).