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. 2011 Dec;31(23):4692–4705. doi: 10.1128/MCB.05979-11

Fig. 4.

Fig. 4.

Ablation of PPARγ-expressing cells causes high bone mass. (A) Schematic diagram of the PTDTA mice. (B and C) μCT analysis of tibiae from PTDTA or control mice (7 months old; female; n = 5). (B) Representative images of the trabecular bone of the tibial metaphysis (top; scale bar, 10 μm) and the entire proximal tibia (bottom; scale bar, 1 mm). (C) Quantification of trabecular bone volume and architecture. The error bars indicate SD. (D) Urinary CTX-1 (n = 5). (E) Serum osteocalcin (n = 5). (F and G) Bone histomorphometry (n = 5). (F) Representative images of TRAP-stained femoral sections. Scale bar, 100 μm. (G) Quantification of osteoclast surface and numbers. (H) PTDTA mice exhibited extramedullary hematopoiesis in the spleen (n = 5). (I and J) Osteoclast differentiation was blunted in the PTDTA culture. (I) Representative images of TRAP-stained osteoclast differentiation culture. Scale bar, 25 μm. (J) Numbers of mature osteoclasts per well (n = 3). (K) Osteoclast precursor proliferation was decreased in the PTDTA culture (n = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.005; ****, P < 0.001; *****, P < 0.0005; n.s., nonsignificant (P > 0.05).