Fig. 6.

NF90 depletion inhibits NHEJ. (A) WCE was mock depleted (Beads) or immunodepleted with Omni, NF90, or DNA-PKcs antibody and then assayed for NHEJ with 5.7-kb substrate. (B) Quantitation of end joining after immunodepletion. Efficiency of dimer formation is expressed as a percentage of input substrate (n = 3, with standard deviations). (C) Immunoblot analysis of depleted extracts. (D) Effect of RNase treatment on NHEJ. Extract was preincubated with EDTA, diluent (−), or RNase A (0.5, 1.5, and 2.5 μg/ml), as indicated. The percent conversion of monomeric substrate to dimer products is shown.