Fig. 7.

NF90 and NF45 depletion increases susceptibility to DNA damage. (A) γ-H2A.X foci in cells depleted of NF90 and NF45. Fluorescence microscopy images of control (c), d3, and d5 cells stained for actin filaments (phalloidin), DNA (Hoechst), and γ-H2A.X (magnification, ×63). (B) Quantitation of γ-H2A.X-positive cells in control and depleted cultures (Total) and in their constituent mononucleated and multinucleated cells. (C) Decreased clonogenicity of depleted cultures after gamma irradiation. Data are presented as percentage survival relative to that of control cells (means of triplicates with standard deviations).