Skip to main content
. 2011 Dec;31(23):4720–4734. doi: 10.1128/MCB.06147-11

Fig. 7.

Fig. 7.

Deacetylation of DNMT1 GK linker reduces DNMT1 methyltransferase-independent transcription repression activity. (A and C) 293T cells in 12-well plates were transfected with either pTK-Luc or pGal4-TK-Luc (0.1 μg), pRL-SV40 (internal control, 0.018 μg), and the indicated HA-DNMT1, Gal4, or Gal4-DNMT1 constructs (0.5 μg). (B) 293T cells in 12-well plates were transfected with pGal4-TK-Luc (0.1 μg), pRL-SV40 (internal control, 0.018 μg), and the indicated Gal4-DNMT1-WT (0.5 μg) or Gal4-16KR (0.5, 1, 1.5, and 2 μg). Firefly luciferase activity is normalized to Renilla luciferase activity and depicted as fold over wild-type value with error bars showing standard deviations from three experiments. Anti-Gal4 and anti-β-actin immunoblot assays were performed to assess Gal4-DNMT1 expression and to serve as loading controls. *, nonspecific bands on Western blots.