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. 2011 Dec;31(23):4801–4813. doi: 10.1128/MCB.05780-11

Fig. 5.

Fig. 5.

Analysis of shRNA-mediated FOSL1 knockdown. (A and B) In vitro analyses. qRT-PCR measurements of Fosl1 mRNA (A) and Western blot analysis of FOSL1 protein (B) in differentiated Rcho-1 TS cells expressing control or Fosl1 shRNAs. The qRT-PCR measurements were performed in quadruplicate. (C to E) In vivo analyses. (C) Western blot analysis of FOSL1 protein in dissected placental samples exposed to control or Fosl1 shRNAs. ACTB was included as a loading control. (D) qRT-PCR analysis of Fosl1 mRNA in dissected placental samples exposed to control or Fosl1 shRNAs. Dashed lines mark the means and the solid lines indicate the SEMs. Sample sizes: control, n = 14; Fosl1 shRNA, n = 12. Fosl1 shRNA treatment significantly decreased Fosl1 mRNA expression (*, P < 0.05). The open circle represents a Fosl1 shRNA sample that escaped knockdown and was not included in the statistical analysis. (E) FOSL1 immunohistochemistry of rat placentation sites exposed to control or Fosl1-specific shRNAs.