Table 1.
Target | Primer or probe | Primer or probe sequence (5′ → 3′) | Probe fluorophore/quencher | Amplicon size (bp) |
---|---|---|---|---|
N. gonorrhoeae genomic | Forward primer | ACCCGATATAATCCGTCCTTCA | 5′-TEX615/3′-IAbRQSp | 72 |
Reverse primer | TTCGGCTCCTTATTCGGTTTAAC | |||
Probe | CGTCCTTCAACATCAGTGAAAATCG | |||
C. trachomatis genomic | Forward primer | ATTTGCCGCTTTGAGTTCTGCTTCCT | 5′-TYE665/3′-IAbRQSp | 75 |
Reverse primer | ATCATAAGGCTTGGTTCAGCAGGATTC | |||
Probe | CCTCCTTGCAAGCTCTGCCTGTGGGG | |||
C. trachomatis plasmid | Forward primer | AGGCGATTTAAAAACCAAGGTCGATGT | 5′-TYE665/3′-IAbRQSp | 89 |
Reverse primer | GAAGAATTGATCCAACACCCTTATCG | |||
Probe | CCGTATGTGAATGTCGAACTCATCCG |