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. 2011 Dec;49(12):4121–4125. doi: 10.1128/JCM.00952-11

Table 1.

Sequences of primers and dually labeled fluorescent probes

Target Primer or probe Primer or probe sequence (5′ → 3′) Probe fluorophore/quencher Amplicon size (bp)
N. gonorrhoeae genomic Forward primer ACCCGATATAATCCGTCCTTCA 5′-TEX615/3′-IAbRQSp 72
Reverse primer TTCGGCTCCTTATTCGGTTTAAC
Probe CGTCCTTCAACATCAGTGAAAATCG
C. trachomatis genomic Forward primer ATTTGCCGCTTTGAGTTCTGCTTCCT 5′-TYE665/3′-IAbRQSp 75
Reverse primer ATCATAAGGCTTGGTTCAGCAGGATTC
Probe CCTCCTTGCAAGCTCTGCCTGTGGGG
C. trachomatis plasmid Forward primer AGGCGATTTAAAAACCAAGGTCGATGT 5′-TYE665/3′-IAbRQSp 89
Reverse primer GAAGAATTGATCCAACACCCTTATCG
Probe CCGTATGTGAATGTCGAACTCATCCG