Table 2.
Yield of HIV genotyping of DBS samples collected in clinical trials in sub-Saharan Africa
| Clinical trial | Sample subset | Median duration (yr) of sample storageb | No. of samplesc | Median log10 HIV viral load (range) | Yield (%) of HIV genotyping usinga: |
Overall recovery (%) with retestingd | |
|---|---|---|---|---|---|---|---|
| Assay #1 | Assay #3 | ||||||
| SWEN | All | 3.3 | 56 | 5.9 (4.0–7.0) | 41/56 (73%) | NA | 53/56 (95%) |
| P1060 | All | 1 | 49 | 5.8 (4.6–6.9) | NA | 38/49 (78%) | 41/49 (84%) |
| Optimal storage | 0.5 | 33 | 6.0 (4.6–6.9) | NA | 29/33 (88%) | 30/33 (91%) | |
| Suboptimal storage | 1.2 | 16 | 5.9 (5.5–6.4) | NA | 9/16 (56%) | 11/16 (69%) | |
a
NA, not applicable.
b
Duration of storage at study sites prior to sample shipment.
c
Samples and sample storage are described in Materials and Methods.
d
Assay #2 was performed if assay #1 or #3 failed to yield genotype (see Materials and Methods).