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Immunohistochemical detection of rotavirus nonstructural proteins. (A) Hepatocyte cytoplasm stained with anti-NSP1 polyclonal antibody. (B) Hepatocyte cytoplasm stained with preimmune rabbit serum. (C) Cardiomyocytes stained with anti-NSP3 polyclonal antibody. (D) Cardiomyocytes stained with preimmune rabbit serum. The reddish-brown color (diaminobenzidine) denotes a positive reaction.
