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Limit of detection. A mixture of A. fumigatus and A. terreus germlings was introduced at low concentrations (7 to 50 germlings) into BAL fluid, followed by nucleic acid extraction. The eluates were tested by each of the Aspergillus PCR assays. The number of copies detected per reaction was plotted against the number of organisms per 0.5 ml BAL fluid for pan-Aspergillus (squares), A. fumigatus (triangles), and A. terreus (circles).
